PE Annexin V Apoptosis Detection Kit with 7-AAD 640934 from BioLegend

Product Specs
ItemPE Annexin V Apoptosis Detection Kit with 7-AAD
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640934
Quantity100 tests
ApplicationsFlow Cytometry (FC)
ReactivityHuman, Mouse, Rat
Antibody TypePrimary
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
TargetAnnexin V
Conjugate/TagPE

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Control of CD4+ T cells to restrain inflammatory diseases via eukaryotic elongation factor 2 kinaseSignal Transduction and Targeted TherapyOctober 24, 2023Hao-Yun Peng, Liqing Wang, Jugal Kishore Das, Anil Kumar, Darby J. Ballard, Yijie Ren, Xiaofang Xiong, Paul de Figueiredo, Jin-Ming Yang, Jianxun Songobtained from BD Bioscience. For apoptosis detection: PE Annexin V apoptosis detection kit with 7-AAD (#640934) from BioLegend (San Diego, CA, USA). For immunofluorescence: eEF2K (#PA5-22175), MitoTracker Deep Red

(2) TRAIL promotes the polarization of human macrophages toward a proinflammatory M1 phenotype and is associated with increased survival in cancer patients with high tumor macrophage contentFrontiers in ImmunologySeptember 21, 2023Sinem Gunalp, Derya Goksu Helvaci, Aysenur Oner, Ahmet Bursalı, Alessandra Conforte, Hüseyin Güner, Gökhan Karakülah, Eva Szegezdi, Duygu SagFACS buffer. Then, cells were incubated in Annexin V Binding Buffer containing Annexin V-PE and 7-AAD (640934; 1:20) according to the manufacturer’s instructions (BioLegend, San Diego, CA) for 15-20 minutes at

(3) Resting natural killer cell homeostasis relies on tryptophan/NAD + metabolism and HIF‐1αEMBO reportsJune 5, 2023Abigaelle Pelletier, Eric Nelius, Zheng Fan, Ekaterina Khatchatourova, Abdiel Alvarado‐Diaz, Jingyi He, Ewelina Krzywinska, Michal Sobecki, Shunmugam Nagarajan, Yann Kerdiles, et al.anti‐Granzyme B (812‐8898, Invitrogen), anti‐Interferon‐gamma (554413, BD), and Annexin V and 7AAD (BioLegend, 640934). Seahorse metabolic flux analysis Freshly isolated NK cells were seeded at 200,000 cells/ well either

(4) Down‐regulation of human long non‐coding RNA LINC01187 is associated with nephropathiesJournal of Cellular and Molecular MedicineMay 1, 2023Theodora Manolakou, Valeria Kaltezioti, Niki Prakoura, Panagiotis Kavvadas, Simone Reichelt‐Wurm, Harikleia Gakiopoulou, Miriam Banas, Bernhard Banas, Maja T. Lindenmeyer, Clemens D. Cohen, et al.apoptosis detection assay, cells were stained with PE Annexin V Apoptosis Detection Kit with 7‐AAD (Cat#640934 Biolegend). The flow cytometer used was FACS‐ARIA‐III (Becton Dickinson Biosciences). GFP+ gated cells

(5) Glucose promotes regulatory T cell differentiation to maintain intestinal homeostasisiScienceSeptember 16, 2022Yu Yu et al.Pierce BCA Protein Assay Kit,ThermoFisher Scientific,Cat#23225 Annexin V/ 7AAD apoptosis kit,Biolegend,Cat# 640934 CellTrace CFSE Cell Proliferation Kit,Invitrogen,Cat#C34554 "ELISA MAXTM Deluxe Sets ELISA kit, Mouse

(6) Differential CXCR4 expression on hematopoietic progenitor cells versus stem cells directs homing and engraftmentJCI insightMay 9, 2022Felker S, Shrestha A, Bailey J, Pillis DM, Siniard D, Malik P.For annexin V and 7AAD staining, PE annexin V Apoptosis Detection Kit with 7-AAD was used (BioLegend, 640934). γH2AX was stained with PE γH2AX (BioLegend, 613411). At 12 weeks following transplant, BM was harvested

(7) CD47 antibody protects mice from doxorubicin-induced myocardial damage by suppressing cardiomyocyte apoptosisExperimental and therapeutic medicineMay 1, 2022Hao Y, Chen L, Jiang Z.cocktail. Apoptotic cells were detected by PE-Annexin V apoptosis detection kit (BioLegend; cat. no. 640934), according to the manufacturer's recommendations. All stained cells were analyzed on a BD FACSAria™

(8) CAR T cells targeting tumor-associated exons of glypican 2 regress neuroblastoma in miceCell reports. MedicineJune 15, 2021Li N, Torres MB, Spetz MR, Wang R, Peng L, Tian M, Dower CM, Nguyen R, Sun M, Tai CH, et al.antibody,Thermo Fisher Scientific,12-2239-42 PE Annexin V Apoptosis Detection Kit with 7-AAD,Biolegend,640934 BV421 mouse anti-human CD45RA antibody,BD Biosciences,562885 APC mouse anti-human CD62L antibody,BD Biosciences

(9) Synthetic Antibody Mimics Based on Cancer-Targeting Immunostimulatory PeptidesChembiochem : a European journal of chemical biologyMay 4, 2021Descalzi-Montoya D, Montel RA, Smith K, Dziopa E, Darwich A, Yang Z, Bitsaktsis C, Korngold R, Sabatino D.and late-stage apoptosis, as well as necrosis using Annexin V/7-AAD stain kit (Biolegend, Catalog No. 640934). The NK92-MI cells (125,000 cells/well) were incubated with and without the activating ligands, synthetic

(10) The transcription factor RelB restrains group 2 innate lymphoid cells and type 2 immune pathology in vivoCellular & molecular immunologyJanuary 1, 2021Zhang L, Ying Y, Chen S, Arnold PR, Tian F, Minze LJ, Xiao X, Li XC.

(11) CX3CR1 But Not CCR2 Expression Is Required for the Development of Autoimmune Peripheral Neuropathy in MiceFrontiers in immunologyJanuary 1, 2021Oladiran O, Shi XQ, Fournier S, Zhang J.46-0081-82",53-6.7 CD11b,1:50,BD,552850,M1/70 CD45,1:50,Biolegend,103116,30-F11 CD62L,1:50,eBioscience,11-0621-85,MEL-14 CD86,1:50,eBioscience,12-0862-82,GL1 CD115,1:50,eBioscience,53-1152-82,AFS98 CCR2,1:50,Biolegend,150604,SA203G11 CX3CR1,1:50,Biolegend,149005,SA011F11 F4/80,1:50,eBioscience,45-4801-82,BM8 Ki67,1:50,Biolegend,652404,SOIA15 CD44,1:50,Biolgend,103030,1M7 CD43,1:50,Biolgend,"121220, 121207",1B11 Annexin V/7AAD kit,1:25,Biolgend,640934, List of antibodies used for FACS.

(12) SHC014748M, a novel selective inhi-bitor of PI3Kδ, demonstrates promising preclinical antitumor activity in B cell lymphomas and chronic lymphocytic leukemiaNeoplasiaDecember 1, 2020Lei Fan, Chao Wang, Liwen Zhao, Zhiqiang Wang, Xian Zhang, Xiaorong Liu, Lei Cao, Wei Xu, Jianyong Licentrifugation for 5 minutes, cells were resuspended in 500 µL of 1 × Annexin V binding buffer (Biolegend, Cat # 640934) and then added with 5 µL of PE Annexin V (Biolegend, Cat # B210905) and 5 µL of 7-AAD viability staining

(13) M. tuberculosis Reprograms Hematopoietic Stem Cells to Limit Myelopoiesis and Impair Trained ImmunityCellOctober 29, 2020Khan N, Downey J, Sanz J, Kaufmann E, Blankenhaus B, Pacis A, Pernet E, Ahmed E, Cardoso S, Nijnik A, et al.M36008 Viability NucSpot Far-Red,Biotium,Cat#40085 PE Annexin V Apoptosis Detection Kit with 7-AAD,BioLegend,Cat# 640934 FOXP3 Transcription Factor Staining Kit,eBioscience,Cat# 00-5523-00 B16-Blue IFN-α/β Cells,Invivogen,Cat#

(14) circRNA-UBAP2 promotes the proliferation and inhibits apoptosis of ovarian cancer though miR-382-5p/PRPF8 axisJournal of ovarian researchJuly 20, 2020Xu Q, Deng B, Li M, Chen Y, Zhuan L.cells/200 ml citrate buffer then Transfer cells to a Falcon tube. PE Annexin V apoptosis detection kit (No. 640934–1, Biolegend, USA) was used by determining the relative amount of AnnexinV-FITC-positive, PI-negative

(15) Acetate coordinates neutrophil and ILC3 responses against C. difficile through FFAR2The Journal of experimental medicineMarch 2, 2020Fachi JL, Sécca C, Rodrigues PB, Mato FCP, Di Luccia B, Felipe JS, Pral LP, Rungue M, Rocha VM, Sato FT, et al.Fixation/Permeabilization Solution Kit with BD GolgiPlug,BD Biosciences,555028 PE Annexin V Apoptosis Detection Kit with 7-AAD,BioLegend,640934 Experimental models: cell lines,Experimental models: cell lines,Experimental models: cell lines HCT-116,"Dr.

(16) Enhanced mitophagy in bronchial fibroblasts from severe asthmatic patientsPloS oneJanuary 1, 2020Ramakrishnan RK, Bajbouj K, Hachim MY, Mogas AK, Mahboub B, Olivenstein R, Hamoudi R, Halwani R, Hamid Q.measured in fibroblasts using the PE Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, Cat. No. 640934), according to manufacturer instructions. Briefly, the cells were labelled with Annexin V-PE and 7-AAD

(17) M1 Macrophage Derived Exosomes Aggravate Experimental Autoimmune Neuritis via Modulating Th1 ResponseFrontiers in immunologyJanuary 1, 2020Du T, Yang CL, Ge MR, Liu Y, Zhang P, Li H, Li XL, Li T, Liu YD, Dou YC, et al.then evaluated for cell apoptosis using PE Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640934) according to manufacturer's instructions. For detection of surface markers on BMDMs, cells were first

(18) CCNB1 Expedites the Progression of Cervical Squamous Cell Carcinoma via the Regulation by FOXM1OncoTargets and therapyJanuary 1, 2020Li S, Liu N, Piao J, Meng F, Li Y.citric acid buffer, which were transferred into Falcon tubes. PE Annexin V Apoptosis Assay Kit (No. 640934–1, BioLegend, San Diego, CA, US) was used to determine the relative number of AnnexinV-fluorescein isothiocyanate

(19) Development of CAR-T Cell Persistence in Adoptive Immunotherapy of Solid TumorsFrontiers in oncologyJanuary 1, 2020Fan J, Das JK, Xiong X, Chen H, Song J.all FITC-, PE-, PE/Cy5-, PE/Cy7, and APC-conjugated antibodies, PE Annexin V Apoptosis Detection Kit (640934) and Cyto-Fast™ Fix/Perm Buffer Set (#426803) were purchased from Biolegend (San Diego, CA) Cell Cultures Tumor

(20) A Novel Role for Triglyceride Metabolism in Foxp3 ExpressionFrontiers in immunologyJanuary 1, 2019Howie D, Ten Bokum A, Cobbold SP, Yu Z, Kessler BM, Waldmann H.a kit from Abcam (ab102918). Apoptotic cells were detected using an Annexin-V/7AAD kit (Biolegend, 640934). Metabolic drugs used in this study were; AZD2988 a DGAT1 inhibitor (Biotechne, 4837), Etomoxir, a

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PE Annexin V Apoptosis Detection Kit with 7-AAD from BioLegend

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PE Annexin V Apoptosis Detection Kit with 7-AAD from BioLegend

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